Code from top papers
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Code: in this repo
a01-PMID-39024031-Code.R
2024 BCMA CAR-T治疗的单细胞数据
优点:细胞分群语句,三个层次细胞命名; sctransform + RPCA 整合多样本; 颜色设置包 paletteer;
缺点:缺少更多细节
a02-argparse/ (run_multiple_seurat.R | utils.R) 来源:群分享
空间转录组 单脚本分析
优点:
R语言的 argparse 传递参数 --input
source() 的使用
缺点:很少注释;没有找到paper
a03-Treg-NC_2021-PMID34599187.Rmd
2021 NC: CD177 modulates the function and homeostasis of tumor-infiltrating regulatory T cells
we identify two distinct transcriptional fates for TI Treg cells, Fate-1 and Fate-2. The Fate-1 signature is associated with a poorer prognosis in ccRCC and several other solid cancers.
优点: Rmd的使用; 多样品整合[MergeSeurat, RunMultiCCA, AlignSubspace] Trajectory 2个命运; 宽变长 melt;
细胞比例图:
```
freq_table <- prop.table(x = table(immune.combined2@ident, immune.combined2@meta.data[, "Type"]),
margin = 2)
freq_table <- as.data.frame(freq_table)
ggplot(freq_table, aes(x=Var1, y=Freq, fill=Var2)) +
geom_bar(stat="identity", position="fill", color="black", lwd=0.25) +
theme(axis.title.x = element_blank())
ggsave("CompositionProp.pdf", width=4, height=2)
```
Code: GitHub
b01-PMID-38200243
2024 Nat Cancer
https://www.nature.com/articles/s43018-023-00691-z
https://github.com/snow55/SIRPAproject/blob/main/Scripts/3_find_DEGs_and_plot.r
优点: 文件编号,带中文注释
b02-PMID-32649887
2020 Cancer Cell: 血液肿瘤的免疫景观
优点: 血液相关
b03-PMID-39047727
2024 Cell: pan-cancer B
code: https://github.com/yuyang3/pan-B
fig: http://pan-b.cancer-pku.cn/
优点: web可视化包 Cirro; Cancer_color_panel 颜色组合;
对亚群做 scVelo https://github.com/yuyang3/pan-B/blob/main/Figure6_trajectory_analysis.ipynb
b04-PMID-39315707
2024 NAR: scMMO-atlas
https://zenodo.org/records/13710871
优点: shiny 界面值得学习,但是没提供代码
b05-PMID-:
Towards modeling context-specific EMT regulatory networks using temporal single cell RNA-Seq data
https://lusystemsbio.github.io/EMT-Cancer-scRnaSeq/EMT-Cancer-scRnaSeq.html 新版本; 老版本 _v1.html
https://github.com/lusystemsbio/EMT-Cancer-scRnaSeq
b06-PMID-37646019:
2023 iScience; CD4T Act vs Rest
https://github.com/JinWLab/T_active
优点: 配色可以参考;
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